The method uses local sequence alignments (BLAST) to find similar sequences (potential homologs) for one or several input sequences. The program extracts all GO terms associated to each of the obtained hits and returns an evaluated GO annotation for the query sequence(s). Enzyme codes are obtained by mapping from equivalent GOs while InterPro motifs can directly be queried at the InterProScan web service. A basic annotation process with Blast2GO PRO consists of 3 steps: blasting, mapping and annotation. These steps will be described in this manual including further explanations and information on additional functions.
Gene Ontology Mapping of Blast results
InterProScan Domain Searches
Various data import and export formats
A. Conesa, S. Götz, J. M. Garcia-Gomez, J. Terol, M. Talon and M. Robles. "Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research", Bioinformatics, Vol. 21, September, 2005, pp. 3674-3676.
A. Conesa and S. Götz. "Blast2GO: A Comprehensive Suite for Functional Analysis in Plant Genomics", International Journal of Plant Genomics, Vol. 2008. 2008, pp. 1-13.
S. Götz et al. "High-throughput functional annotation and data mining with the Blast2GO suite", Nucleic Acids Research, Vol. 36, June, 2008, pp. 3420-3435.
S. Götz et al. "B2G-FAR, a species centered GO annotation repository", Bioinformatics, Vol. 27 (7), 2011, pp. 919-924.