Mix-Square Transcript Quantification plug-in yields highly accurate concentration estimates for gene isoforms by adapting to the positional coverage bias in RNA-Seq data.
This leads to higher accuracy in the detection of differential expression of genes and gene isoforms. Mix-Square enables repeatable concentration estimates across multiple library preparations and sequencing facilities and can be used as an explorative tool to investigate the positional biases present in RNA-Seq data.
Precise transcript concentration estimates
Accurate detection of differential expression
Exceptional reproducibility across variable conditions